T7

Part:BBa_K2207025:Design

Designed by: Yuxing Chen   Group: iGEM17_ZJU-China   (2017-10-23)


Modified T7 promoter 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is quite short. So we recommend PCR to assembly it with protein coding sequence.


Source

The original sequence of the T7 promoter is from BBa_K525998, and we adapted PCR to induce the site mutation.

References